METHOD OF PRODUCTION OF MONOCLONAL ANTIBODIES TO PROTEIN P30 OF AFRICAN SWINE FEVER VIRUS USING RECOMBINANT CONSTRUCTIONS Russian patent published in 2014 - IPC C12N5/20 C07K16/08 

FIELD: biotechnology.

SUBSTANCE: invention consists in priming of mice with plasmid DNA encoding the gene of protein of interest to the researcher, which provides in vivo expression, processing, corresponding post-translational 3D conformation and induces an immune response similar to the serum to the infectious agent. Synergism of priming with DNA-plasmid and booster of effect of administration of recombinant protein in soluble form provides high avidity of CD4+ and CD8+ T-cells and, as a consequence, the affinity and avidity of monoclonal antibodies obtained. Application of DNA immunisation method and subsequent administration of a recombinant protein expands the range of methods of production of hybridomas producing specific mAb to different antigens. The method was tested in preparation of monoclonal antibodies to epitopes of phosphoprotein vp30 of African swine fever virus. The monoclonal antibodies thus obtained are capable of recognising antigenic determinants of phosphoprotein vp30 of African swine fever virus with cross immunoreactivity to the recombinant protein produced in E.coli clone PTT9/ASFVp30/2.

EFFECT: increased yield of hybridomas producing mAb to the desired antigen and reduced time of receiving donors of immune lymphocytes and lines of hybridomas producing specific mAb.

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