FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and veterinary virology. Disclosed is an expression plasmid vector, providing production of recombinant protein in E. coli cells, consisting of african swine fever virus (ASFV) protein p30, thioredoxin and polyhistidine sections.
EFFECT: invention can be used for making virus-specific antigens for analyzing swine serums by immunoblotting.
1 cl, 3 dwg, 1 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| BACTERIAL STRAIN OF ESCHERICHIA COLI KRX pET32b/ASFV/p30-PRODUCER OF CHIMERICAL RECOMBINANT PROTEIN p30 OF AFRICAN SWINE FEVER VIRUS | 2017 |
|
RU2647573C1 |
| Ecoli BL21 (DE3)pLysS CELL STRAIN, pTT9/ASFVp30 CLONE CONTAINING RECOMBINANT PLASMID WITH INTEGRATION OF PART OF CP204L GENE OF AFRICAN SWINE FEVER VIRUS, ENCODING CONFORMATIONAL EPITOPE OF PROTEIN p30, FOR PRODUCING DIAGNOSTIC PREPARATIONS | 2011 |
|
RU2463343C1 |
| OLIGONUCLEOTIDE PRIMERS FOR PRODUCTION OF LIBRARY OF FULL-LENGTH GENES ENCODING IMMUNODOMINANT PROTEINS Gn, Gc AND N OF RIFT VALLEY FEVER VIRUS | 2013 |
|
RU2555527C2 |
| GENETIC CONSTRUCT FOR GENE EXPRESSION mNG_CD4-CXCR4, RECOMBINANT PLASMID rVSV_MNG_CD4-CXCR4 AND A RECOMBINANT VESICULAR STOMATITIS VIRUS STRAIN rVSV_mNG_CD4-CXCR4, WHICH PROVIDES TARGETED VIROLYSIS OF CELLS EXPOSING HIV-1 GP120/GP41 PROTEINS TROPISM X4 ON THEIR SURFACE | 2021 |
|
RU2768032C1 |
| OPTIMIZED RECOMBINANT PROTEIN ENCODING GENE - HUMAN INTERFERON BETA ANALOG | 2015 |
|
RU2614264C2 |
| RECOMBINANT PLASMID DNA CODING FUSION PROTEIN L-HEP, ESCHERICHIA COLI STRAIN PRODUCER OF SAID PROTEIN AND METHOD OF PRODUCING RECOMBINANT PROTEIN | 2019 |
|
RU2716975C1 |
| GENETIC CONSTRUCT FOR EXPRESSION OF mNG_CD4-CCR5 GENES, RECOMBINANT PLASMID rVSV_mNG_CD4-CCR5 AND RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS rVSV_mNG_CD4-CCR5 PROVIDING TARGETED VIROLYSIS OF CELLS DISPLAYING HIV-1 PROTEINS GP120/GP41 OF R5 TROPISM ON THEIR SURFACE | 2021 |
|
RU2769125C1 |
| KIT OF OLIGONUCLEOTIDE PRIMERS FOR GENERATION OF LIBRARY OF FULL-LENGTH GENES CODING IMMUNODOMINANT PROTEINS P17 (D11L), P30 (CP204L), P54 (E183L), P60 (CP530R), P72 (B646L) OF AFRICAN SWINE FEVER VIRUS | 2014 |
|
RU2571855C2 |
| RECOMBINANT VECTOR CONTAINING THE IMMUNOGENIC PROTEIN OF THE AFRICAN SWINE FEVER VIRUS, RECOMBINANT BACTERIA AND THEIR APPLICATION | 2020 |
|
RU2771177C1 |
| RECOMBINANT PLASMID VECTOR pET32-WNV-DIII, PROVIDING SYNTHESIS AND SECRETION OF RECOMBINANT DOMAIN III OF STRUCTURAL GLYCOPROTEIN E OF WEST NILE VIRUS IN ESCHERICHIA COLI CELLS, ESCHERICHIA COLI BL21(DE3)-DIII-WNV CELL LINE STRAIN AND DIII-WNV RECOMBINANT PROTEIN FOR PREPARING IMMUNOBIOLOGICAL PREPARATIONS | 2024 |
|
RU2821341C1 |
