FIELD: biotechnology.
SUBSTANCE: strain of Escherichia coli pET32b/KRX ASFV/p30 cells, obtained by transformation of a stable strain of E.coli KRX pET32b/ASFV/p30 by plazmid vector pET32b(+)ASFV/p30e2 and deposited in the State collection of strains of microorganisms of State Research Institution National Research Institute for Veterinary Virology and Microbiology (SRNRIVVM) of the Russian Academy of Agricultural Science under No. "734-МБШ". The proposed strain is stably expressed by chimerical recombinant protein p30 of African swine fever virus, consisting of antigen-active p30 fragment, leading sequence of thioredoxin (Trx-Tag) and two poly-histidine areas (6xHis).
EFFECT: proposed strain allows you to produce an electrophoretically and immunochemical clean antigen-active chimerical recombinant protein p30, which can be used in testing systems, designed for African swine fever serodiagnosis, in particular, using the immunoblotting method.
2 dwg, 1 tbl, 3 ex
| Title |
Year |
Author |
Number |
| EXPRESSION PLASMID VECTOR PET32B(+)/ASFV/P30E2 FOR PRODUCTION OF RECOMBINANT PROTEIN, CONSISTING OF AFRICAN SWINE FEVER VIRUS PROTEIN P30, THIOREDOXIN AND POLYHISTIDINE SECTIONS |
2015 |
- Sereda Aleksej Dmitrievich
- Imatdinov Ilnaz Ramisovich
- Kazakova Anna Sergeevna
- Imatdinov Almaz Ramisovich
- Dubrovskaja Olga Aleksandrovna
|
RU2603056C2 |
| METHOD OF PRODUCTION OF MONOCLONAL ANTIBODIES TO PROTEIN P30 OF AFRICAN SWINE FEVER VIRUS USING RECOMBINANT CONSTRUCTIONS |
2013 |
- Pershin Andrej Sergeevich
- Kazakova Anna Sergeevna
- Kapustina Ol'Ga Vladimirovna
- Vlasova Natal'Ja Nikiforovna
|
RU2534343C1 |
| Ecoli BL21 (DE3)pLysS CELL STRAIN, pTT9/ASFVp30 CLONE CONTAINING RECOMBINANT PLASMID WITH INTEGRATION OF PART OF CP204L GENE OF AFRICAN SWINE FEVER VIRUS, ENCODING CONFORMATIONAL EPITOPE OF PROTEIN p30, FOR PRODUCING DIAGNOSTIC PREPARATIONS |
2011 |
- Kopytov Valerij Olegovich
- Tsybanov Sodnom Zhem'Janovich
- Kazakova Anna Sergeevna
- Juzhuk Tat'Jana Ehmmanuilovna
- Beljanin Sergej Aleksandrovich
- Guzalova Anna Grigor'Evna
- Vlasova Natal'Ja Nikiforovna
- Kolbasov Denis Vladimirovich
|
RU2463343C1 |
| KIT OF OLIGONUCLEOTIDE PRIMERS FOR GENERATION OF LIBRARY OF FULL-LENGTH GENES CODING IMMUNODOMINANT PROTEINS P17 (D11L), P30 (CP204L), P54 (E183L), P60 (CP530R), P72 (B646L) OF AFRICAN SWINE FEVER VIRUS |
2014 |
- Mima Ksenija Aleksandrovna
- Burmakina Galina Sergeevna
- Titov Il'Ja Andreevich
- Malogolovkin Aleksandr Sergeevich
|
RU2571855C2 |
| ATTENUATED ASFV/CV60/2020 STRAIN OF AFRICAN SWINE FEVER VIRUS OF THE ASFARVIRIDAE FAMILY, GENUS ASFIVIRUS FOR THE STUDY OF IMMUNOLOGICAL REACTIONS, MOLECULAR GENETIC ANALYSIS, GENETIC MODIFICATION AND CREATION OF A PROTOTYPE VACCINE AGAINST ASF |
2021 |
- Vlasova Natalya Nikiforovna
- Igolkin Aleksej Sergeevich
- Mazlum Ali
- Pershin Andrej Sergeevich
- Zhukov Ivan Yurevich
- Shevchenko Ivan Vyacheslavovich
- Shotin Andrej Romanovich
- Elsukova Aleksandra Andreevna
- Aronova Elena Vladimirovna
|
RU2760399C1 |
| RECOMBINATIONAL CARTRIDGE CONTAINING GENES EP153R AND EP402R OF STRAIN F-32 OF AFRICAN SWINE FEVER VIRUS AND RECOMBINANT STRAIN DSWCONGO/FRANCELECTINCD2 OF AFRICAN SWINE FEVER VIRUS |
2014 |
- Burmakina Galina Sergeevna
- Morgunov Jurij Petrovich
- Titov Il'Ja Andreevich
- Mima Ksenija Aleksandrovna
- Kolbasov Denis Vladimirovich
- Malogolovkin Aleksandr Sergeevich
|
RU2571858C1 |
| OLIGONUCLEOTIDE PRIMERS FOR PRODUCTION OF LIBRARY OF FULL-LENGTH GENES ENCODING IMMUNODOMINANT PROTEINS Gn, Gc AND N OF RIFT VALLEY FEVER VIRUS |
2013 |
- Imatdinov Il'Naz Ramisovich
- Balysheva Vera Ivanovna
|
RU2555527C2 |
| ACHS/VNIIZZH/CV-1 STRAIN OF AFRICAN SWINE FEVER, WITH DECREASED VIRULENCE FOR PIGS, FOR VIROLOGIC, DIAGNOSTIC, MOLECULAR-GENETIC AND SURVEILLANCE STUDIES |
2018 |
- Vlasova Natalya Nikiforovna
- Zhukov Ivan Yurevich
- Mazlum Ali
- Sharypova Darya Viktorovna
- Pershin Andrej Sergeevich
- Igolkin Aleksej Sergeevich
|
RU2675535C1 |
| OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT PROBE WITH INNER DAMPER, COMPLEMENTARY TO THE GENE SECTION P30 (CP204L) OF THE AFRICAN SWINE FEVER VIRUS, FOR USE IN POLYMERASE CHAIN REACTION IN REAL TIME |
2016 |
- Kudryashov Dmitrij Andreevich
- Burmakina Galina Sergeevna
- Titov Ilya Andreevich
- Tsybanov Sodnom Zhamyanovich
- Malogolovkin Aleksandr Sergeevich
|
RU2606253C1 |
| RECOMBINATIONAL CARTRIDGE CONTAINING THE EP153R AND EP364R GENES OF THE CONGO (KK-262) STRAIN OF THE AFRICAN SWINE FEVER VIRUS AND RECOMBINANT ΔCONGOCD2V STRAIN OF THE AFRICAN SWINE FEVER VIRUS |
2016 |
- Burmakina Galina Sergeevna
- Morgunov Yurij Petrovich
- Titov Ilya Andreevich
- Mima Kseniya Aleksandrovna
- Kolbasov Denis Vladimirovich
- Malogolovkin Aleksandr Sergeevich
|
RU2654586C2 |
