METHOD OF PRODUCTION OF HYDROLYZATE FROM FRUIT AND SKIN OF BANANAS AS GROWTH STIMULATOR FOR CULTIVATION OF LEPTOSPIRA Russian patent published in 2014 - IPC C12P21/06 C12N1/00 

FIELD: biotechnology.

SUBSTANCE: banana fruit and skin are weighed, washed, crushed and mixed with water preheated to 45±1°C in the ratio of 1:3. It is alkalised to pH 8.2-8.3, the enzyme is added, which is used as the pancreas of cattle in the amount of 9-15% of total volume. The preservative is added, which is used as chloroform in an amount of 1% of total volume. The mixture is subjected to hydrolysis for 10 days at a temperature of 45-50°C. During the first 24 hours the mixture is stirred every 15 minutes for 5 minutes, and then every 2 hours for 5 minutes. The hydrolyzate is filtered through cheesecloth, then the filter towels (paper) and sterilised by ozonation. It is covered with cork and stored hermetically sealed at a temperature of from 2°C to 8°C.

EFFECT: invention provides obtaining of transparent hydrolyzate with increased sterility, with high biological activity, which leads to improvement of its properties when it is used as a growth stimulator of Leptospira.

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