FIELD: biotechnologies.
SUBSTANCE: invention describes a strain of cultivated hybrid animal cells Mus musculus L. CCHFV Vd-1. The strain is obtained by hybridisation of two types of cells: splenocytes of an inbred mouse of BALB/c line, which is immunised with an antigene of Crimean-Congo hemorrhagic fever virus (CCHF), and cells of mouse myeloma Sp 2/0. The strain - IgG1 MCA producer to CCHF virus is called CCHFV Vd-1 (4G4/B6). The strain is deposited in the State Collection of Pathogenic Microorganisms and Cell Cultures "GKPM-OBOLENSK" and is provided with number H-25. The strain is a producer of raw material for preparation of a diagnostic product for a method of fluorescent antibodies, as well as a source for obtaining one of the components of a set of monoclonal reagents for immunoenzymometric detection of antigens of CCHF virus.
EFFECT: invention can be used in medical practice to detect Crimean-Congo hemorrhagic fever virus in clinic or sectional specimens to specify a diagnosis and to solve scientific and research tasks on study of properties of CCHF virus and on creation of diagnostic preparations against Crimean hemorrhagic fever.
1 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| STRAIN OF CULTIVATED HYBRID CELLS OF ANIMAL MUS MUSCULUS L CCHFV Vd-3-PRODUCER OF MONOCLONAL ANTIBODY 3H6/F2 TO VIRUS OF CRIMEAN-CONGO HAEMORRHAGIC FEVER | 2013 |
|
RU2528869C1 |
| STRAIN OF CULTIVATED HYBRID CELLS OF ANIMAL MUS MUSCULUS L CCHFV Vd-2-PRODUCER OF MONOCLONAL ANTIBODY 1E2/E5 TO VIRUS OF CRIMEAN-CONGO HAEMORRHAGIC FEVER | 2013 |
|
RU2528868C1 |
| STRAIN OF HYBRID ANIMAL CELLS Mus musculus L - PRODUCER OF MONOCLONAL ANTIBODIES FOR EXPOSING VP40 PROTEIN OF EBOLA VIRUS, ZAIRE SUBTYPE (Mainga STRAIN) (VERSIONS), MONOCLONAL ANTIBODY PRODUCED BY SAID STRAIN (VERSIONS) AND SET FOR "SANDWICH" FORMAT IMMUNOENZYMOMETRIC TEST SYSTEM FOR EXPOSING VP40 PROTEIN OF EBOLA VIRUS, ZAIRE SUBTYPE (Mainga STRAIN) | 2008 |
|
RU2395577C1 |
| STRAIN OF HYBRID ANIMAL CELLS MUS MUSCULUS L3F9 - PRODUCER OF MONOCLONAL ANTIBODIES SUITABLE FOR USE IN "SANDWITCH" IMMUNOENZYMOMETRIC SYSTEM FORMAT FOR DETECTING VP35 PROTEIN OF MARBURG VIRUS AND 3F9 MONOCLONAL ANTIBODIES PRODUCED BY SAID STRAIN OF HYBRID CELLS | 2008 |
|
RU2393220C1 |
| STRAIN OF HYBRID ANIMAL CELLS Mus musculus L - PRODUCER OF MONOCLONAL ANTIBODIES FOR EXPOSING VP40 PROTEIN OF MARBURG VIRUS, (Popp STRAIN) (VERSIONS), MONOCLONAL ANTIBODY PRODUCED BY SAID STRAIN (VERSIONS) AND SET FOR "SANDWICH" FORMAT IMMUNOENZYMOMETRIC SYSTEM FOR EXPOSING VP40 MATRIX PROTEIN OF MARBURG VIRUS (Popp STRAIN) | 2008 |
|
RU2395575C1 |
| STRAIN OF CULTIVATED HYBRID CELLS OF ANIMAL Mus musculus Bpm Vd-8 - PRODUCER OF MONOCLONAL ANTIBODY 3C/A TO ANTIGEN 200 kDa OF PSEUDOCHOLERA ACTIVATOR | 2014 |
|
RU2555544C1 |
| STRAIN OF HYBRID ANIMAL CELLS Mus museums L - PRODUCER OF MONOCLONAL ANTIBODIES FOR EXPOSING NUCLEOPROTEIN OF EBOLA VIRUS, ZAIRE SUBTYPE (Mainga STRAIN) (VERSIONS), MONOCLONAL ANTIBODY PRODUCED BY SAID STRAIN (VERSIONS) AND SET FOR "SANDWICH" FORMAT IMMUNOENZYMOMETRIC TEST SYSTEM FOR EXPOSING NUCLEOPROTEIN OF EBOLA VIRUS, ZAIRE SUBTYPE (Mainga STRAIN) | 2008 |
|
RU2395576C1 |
| STRAIN OF CULTIVATED HYBRID ANIMAL CELLS Mus musculus Bpm Vd-9 - PRODUCER OF MONOCLONAL ANTIBODY 5C/F/C TO ANTIGEN 200 kDa MELIOIDOSIS AGENT | 2014 |
|
RU2556803C1 |
| STRAIN OF CULTIVATED HYBRID ANIMAL CELLS MUS MUSCULUS L BPM VD-11 - PRODUCERS OF MONOCLONAL ANTIBODY 6A/G TO ANTIGEN 200 kDA OF MELIOIDOSIS PATHOGEN | 2014 |
|
RU2570634C1 |
| STRAIN OF CULTIVATED HYBRID ANIMAL CELLS MUS MUSCULUS L BPM VD-10 - PRODUCER OF MONOCLONAL ANTIBODY 5H/E TO ANTIGEN KDA 200 OF MELIOIDOSIS PATHOGEN | 2014 |
|
RU2570638C1 |
