FIELD: chemistry.
SUBSTANCE: invention relates to a method of lyophilisation of a composition, which contains purified antithrombin III (AT III) and a crystallised substance, selected from alanine, mannitol, glycine or NaCl. The claimed method includes freezing the composition at a temperature from -52°C to -60°C for 6-15 hours, annealing the composition at -30°C for 1 hour, re-freezing the composition at a temperature from -52°C to -60°C for 2-15 hours at keeping the product temperature between -48°C and -52.7°C for 4-10 before lyophilisation and drying the composition with obtaining a lyophilised cake. The invention also relates to a pharmaceutical set, which contains the said lyophilised cake and a liquid reagent.
EFFECT: invention provides obtaining the lyophilised composition, containing AT III, which preserves its activity and stability.
14 cl, 24 dwg, 5 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| THROMBIN SOLUTION AND METHODS FOR USE THEREOF | 2013 |
|
RU2670956C9 |
| STABILISED IX FACTOR AND TREGALOSE- CONTAINING COMPOSITIONS | 2008 |
|
RU2481823C2 |
| METHOD OF PRODUCING CRYSTALS OF A DIAZABICYCLOOCTANE DERIVATIVE AND A STABLE LYOPHILIZED PREPARATION | 2015 |
|
RU2732129C2 |
| LYOPHILIZED PHARMACEUTICAL COMPOSITIONS | 2016 |
|
RU2723590C2 |
| LYOPHILISATION OF SYNTHETIC LIPOSOMAL PULMONARY SURFACTANT | 2013 |
|
RU2671500C2 |
| LYOPHILISED PREPARATION OF CYTOTOXIC DIPEPTIDES | 2012 |
|
RU2597154C2 |
| LIOPHILIZED LIPOSOMES | 2012 |
|
RU2648753C2 |
| LYOPHILIZED LIPOSOMES | 2012 |
|
RU2780489C2 |
| SOLID PHARMACEUTICAL COMPOSITIONS CONTAINING BIOPTERIN DERIVATIVES, AND METHODS OF USING SUCH COMPOSITIONS | 2015 |
|
RU2694368C2 |
| COMPOSITIONS CONTAINING ANTIBODY-DRUG CONJUGATES DUOCARMICIN | 2016 |
|
RU2687237C1 |
