METHOD OF LYSOCYME COVALENT IMMOBILIZATION FOR SUBSEQUENT APPLICATION OF IMMOBILIZED LYSOZYME TO REDUCE BACTERIAL POPULATION OF BIOLOGICAL FLUIDS Russian patent published in 2019 - IPC C12N9/36 C12N11/10 G01N33/573 

FIELD: medicine.

SUBSTANCE: invention relates to technologies for production and use of sorbents used for medical purposes, namely for extracorporeal therapy of patients with sepsis using biological liquids sorption. Objective of invention is to widen the range of methods for efficient preparation and antibacterial application in composition of sorbent covalently immobilized lysozyme with no risk of lysozyme leakage into bioliquid (aqueous solution, including saline solution, as well as blood plasma and whole blood (taking into account sorbent hemocompatibility)). Objective is solved by the proposed method of lysocyme covalent immobilization on an aminated agarose matrix, namely: lysocyme immobilization on an aminated agarose matrix to obtain a molecular spacer -NH-C₆H₁₂-NH-C₅H₁₀-NH-, attaching a lysocyme molecule. Matrix of hemocompatible sorbent used is commercially available macroporous agarose matrices Sepharose (manufactured by GE Healthcare, USA) and WorkBeads (WB) 200 Sec (manufactured by Bio-Works, Sweden).

EFFECT: immobilized lysozyme in the composition of the obtained sorbent with no risk of lysozyme leakage is used to reduce bacterial contamination of biological fluids by lysing bacterial cells with observing the lysis results in two ways: statically tracking the optical density drop or dynamically in the flow on the sorption column.

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