FIELD: biotechnology.
SUBSTANCE: method of production of bacteriologically pure cultures of marine blue-green algae provides chemical sterilisation of microalgae cultures by treatment them in the solution of sterile sea water containing 0.1% phenol and 1.0% ethyl alcohol. Microalgae are kept in sterile sea water containing phenol and ethyl alcohol in a predetermined ratio for 4-5 hrs. Irradiation with UV light is carried out for 9-10 minutes. The treatment of microalgae with antibiotics and fungicide is carried out. At that the antibiotics are used as chloromycetin and ampicillin in the amount of 50 µg/ml each, and fungicide is used as nystatin in the amount of 25 µg/ml.
EFFECT: invention enables to improve the efficiency of extraction of bacteriologically pure culture of blue-green microalgae.
1 tbl, 20 ex
| Title | Year | Author | Number |
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RU2102870C1 |
