FIELD: veterinary medicine.
SUBSTANCE: presented subline of cells A4C2/9k is highly sensitive to the ASF virus. The growth medium is used as medium Needle-MEM with 10% blood serum of swine. The inoculating concentration is 80-100 thousand cells/ml. The mitotic index to 2-3 days of cultivation is 25-35. The subline of cells is deposited at the Specialized collection of cell cultures of agricultural and game animals at the All-Russian research institute of experimental veterinary medicine under the number of 87.
EFFECT: invention enables to isolate the African swine fever virus without prior adaptation in reaction of hemadsorption and provides its accumulation in titre.
1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
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| ATTENUATED STRAIN "MSC-2015 VNIIVViM" OF AFRICAN SWINE FEVER VIRUS SEROTYPE VIII FOR VIROLOGY AND MOLECULAR-GENETIC ANALYSIS | 2016 |
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| STRAIN "STAVROPOL 01/08" OF AFRICAN SWINE PLAGUE VIRUS FOR VIROLOGICAL, MOLECULAR-GENETIC AND MONITORING RESEARCH | 2010 |
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| STRAIN OF INTRASPECIES HYBRID CELLS SUIS DOMESTICA USED FOR ISOLATION AND CULTURING PIG PLAGUE CLASSIC VIRUS | 1994 |
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| METHOD FOR DETERMINING ANTIGEN AFFINITY OF HEMABSORBING ISOLATES OF AFRICAN PIG PEST VIRUSES | 1996 |
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| TCh (TESTIS CAPRA hircus), TRANSPLANTABLE MONOLAYER SUBLINE OF TESTICLE CELLS OF A MONTH-OLD GOATLING INTENDED FOR REPRODUCTION OF SMALLPOX VIRUSES, PLAGUE OF SMALL RUMINANTS AND INFECTIOUS BOVINE NODULAR DERMATITIS, AS WELL AS FOR MAKING DIAGNOSTIC AND PREVENTIVE VETERINARY BIOPREPARATIONS | 2021 |
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| STRAIN OF EWE (OVIS ARIES) INTRASPECIES HYBRID KIDNEY CELLS WITH EWE SPLENOCYTES FOR VIRUS CULTURING | 2001 |
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