FIELD: biotechnology.
SUBSTANCE: characterised strain was isolated from diseased pigs and produced by serial passages on sensitive hetero- and homologous cell cultures and deposited in the collection of the FSBI "Federal Animal healthcare centre" under the registration number of FMD virus strain A No.2187/Kuti/2013 (production). The presented strain is reproduced in monolayer cell culture of porcine kidney (PK), passaged cell cultures of kidney of Siberian mountain ibex (SMIK-30), VPK-21 and IB-RS-2. During 18÷24 hours of incubation the virus yield in the said cell cultures reaches the values of 6.0÷7.0 lg TCD50/cm3. At high multiplicity of infection (1÷10 TCD/cell) causes TCID50 after 5 hours, while maintaining the original characteristics when passaging in cell cultures for 5 passages.
EFFECT: invention can be used to monitor the antigenic and immunogenic activity and for producing biological products for diagnostics and specific prophylaxis of FMD of type A.
6 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| INACTIVATED SORBED VACCINE AGAINST FMD OF TYPE A | 2014 |
|
RU2563345C1 |
| A N2269/VNIIZZH/2015 STRAIN OF TYPE A APHTAE EPIZOOTICAE FOOT-AND-MOUTH DISEASE VIRUS FOR CONTROL OF ANTIGENIC AND IMMUNOGENIC ACTIVITY AND FOR MANUFACTURE OF BIOPREPARATIONS FOR DIAGNOSIS AND SPECIFIC PREVENTION OF TYPE A FOOT-AND-MOUTH DISEASE | 2016 |
|
RU2640261C1 |
| INACTIVATED SORPTIVE FOOT-AND-MOUTH DISEASE TYPE A VACCINE | 2017 |
|
RU2681815C1 |
| STRAIN A №2155/BAIKAL/2013 OF MURRAIN VIRUS APHTAE EPIZOOTICAE TYPE A FOR CONTROL OF ANTIGENIC AND IMMUNOGENIC ACTIVITY AND TO PRODUCE BIOPREPARATIONS FOR DIAGNOSTICS AND SPECIFIC PREVENTION OF MURRAIN TYPE A | 2015 |
|
RU2603255C9 |
| INACTIVATED SORPTIVE VACCINE TO FMD TYPES A, O, ASIA-1 | 2015 |
|
RU2603003C1 |
| INACTIVATED EMULSION VACCINE AGAINST FOOT-AND-MOUTH DISEASE TYPE A | 2017 |
|
RU2665850C1 |
| FOOT-AND-MOUTH DISEASE VIRUS STRAIN Aphtae epizooticae TYPE A FOR PRODUCING AND CONTROLLING BIOLOGICAL PREPARATION FOR DIAGNOSTICS AND SPECIFIC PREVENTION OF TYPE A FOOT-AND-MOUTH DISEASE | 2014 |
|
RU2560268C1 |
| INACTIVATED EMULSION VACCINE AGAINST FOOT-AND-MOUTH DISEASE TYPES A, O, ASIA-1 | 2015 |
|
RU2593718C1 |
| STRAIN A №2166/KRASNODARSKIY/2013 MURRAIN VIRUS APHTAE EPIZOOTICAE TYPE A FOR ANTIGENIC AND IMMUNOGENIC ACTIVITY AND TO PRODUCE BIOPREPARATIONS FOR DIAGNOSIS AND SPECIFIC PREVENTION OF MURRAIN TYPE A | 2015 |
|
RU2604200C9 |
| A 2045/KYRGYZSTAN/ 2007 STRAIN OF FOOT-AND-MOUTH DISEASE VIRUS Aphtae epizooticae TYPE A FOR ANTIGENIC AND IMMUNOGENIC PERFORMANCE CONTROL OF FOOT-AND-MOUTH DISEASE VACCINES AND FOR PRODUCTION OF BIOPREPARATIONS FOR DIAGNOSIS AND SPECIFIC PREVENTION OF FOOT-AND-MOUTH DISEASE TYPE A | 2010 |
|
RU2451745C2 |
