FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology, in particular to obtaining protein scaffolds bound with IgG, and can be used in diagnosis or treatment. The method includes obtaining a protein scaffold based on a consensus sequence of the tenth fibronectin type III (FN3) repeat from human fibronectin, which contains amino acid sequence SEQ ID NO: 16. The obtained protein scaffold is used to create a protein scaffold library by introducing diversity into copies of said polypeptide by mutating at least one loop region of residues 22-28 and 75-81 of SEQ ID NO:16.
EFFECT: invention enables to obtain a protein scaffold with high thermal stability and low risk of autoimmune response when introduced into the body.
2 cl, 8 dwg, 3 tbl, 3 ex
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