METHOD OF CULTURING Listeria monocytogenes ON NUTRIENT MEDIUM PREPARED ON BASIS LEAF LETTUCE (Lactuca sativa) Russian patent published in 2015 - IPC C12N1/20 C12R1/01 

FIELD: biotechnology.

SUBSTANCE: method of culturing listerious microbe comprises preparing the nutrient medium containing the mother solution of leaf lettuce juice, agar and phosphate buffer in a predetermined ratio. On the prepared nutrient medium Listeria monocytogenes is applied and cultured at a temperature of 20-22°C for least 24 hours. At that the mother solution of leaf lettuce juice is prepared by sequential washing it with tap water and sterile water, followed by drying at room temperature, grinding and squeezing of juice from the resulting mass.

EFFECT: invention enables to improve the accuracy of detection of Listeria, and to simplify the method of cultivation.

2 cl, 3 dwg, 1 tbl, 6 ex