FIELD: biotechnology, microbiology.
SUBSTANCE: invention proposes a medium consisting of two layers - backing agar and covering agar. Backing agar comprises agar "Difko" and physiological solution. Covering agar comprises agar "Difko", yeast extract, dithiothreitol rabbit fibrin-free blood, sodium chloride, and beef-extract broth or Hottinger broth as a nutrient base. Invention provides study the listeriolysin expression level, to carry out total screening and differentiation of Listeria strains population based on feature of their hemolytic activity. Invention can be used in bacteriological diagnosis of listeriosis in research aims for characterization of strains by their biological activity.
EFFECT: valuable properties of medium.
3 tbl, 2 dwg, 3 ex
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