FIELD: medicine.
SUBSTANCE: raw material is sea urchin's (Strongylocentrotus intermedius) shell and spines, which are defrosted or separated from a preserving agent by decantation, washed in water, defatted by ethanol, dried, ground, demineralised by concentrated phosphoric acid and added with distilled water, extracted three times in ethanol; the produced extract is passed through a chitosan column; the column effluent is boiled off; the residue is dissolved in distilled water, filtered, passed through a polychrome-1 column; the column is washed with distilled water; the end product is eluted with an aqueous solution of ethanol, evaporated to dryness, dissolved in ethyl acetate; the end product is deposited by hexane, filtered and dried.
EFFECT: extending the range of biologically active substances produced by industrial processing of commercial sea urchins (S intermedius) that makes it possible to produce an individual compound - spinochrome B, which is an effective antioxidant and exhibits the antiallergic properties.
3 dwg, 1 ex
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Authors
Dates
2015-11-20—Published
2014-12-11—Filed