METHOD FOR SYNTHESIS OF 2,3,6,7-TETRAHYDROXYNAPHTHAZARIN Russian patent published in 2011 - IPC A61K31/122 

FIELD: chemistry.

SUBSTANCE: invention relates to the technology of processing natural objects. The material used to produce 2,3,6,7-tetrahydroxynaphthazarin (spinochrome E) is wastes from processing sea urchins Strongylocentrotus nudus, which are defrosted or separated from a preserving agent through decantation. The raw material is washed with water, degreased with 96% ethyl alcohol and the extracted three times with 96% ethyl alcohol with addition of phosphoric acid (pH 3.0-3.5) with ratio raw material: extraction agent equal to 1.0:(1.0-1.2) for 12-24 hours.The obtained extract is passed through a column with chitosan. The column is washed with 96% ethyl alcohol and pigments are then eluted with 96% ethyl alcohol with addition of hydrochloric acid (pH 2-4). Further, the eluate is passed through a column with polychrome-1. The column is washed with distilled water and the end product is eluted with 40-50% aqueous solution of ethyl alcohol. The solvent is distilled in a vacuum and the water residue is lyophilised. Spinochrome E is the sublimated at 195-200°C or recrystallised from ethyl alcohol.

EFFECT: method widens the range of biologically active substances obtained from wastes of industrial processing of sea urchins.

3 dwg, 2 ex