FIELD: medicine.
SUBSTANCE: simulation procedure involves Wistar rats. The procedure uses mature female rats with a regular oestrus cycle aged 120 days or more and 180-210 gram heavy. In the dioestrus phase proven by cytological analysis of vaginal smears, 10 mcl of the solution (2×10-3 mol) of kisspeptinergic receptor blocker - kisspeptin-234 trifluoroacetate salt is introduced into nasal passages. The anovular phase prolongation is proven by cytological analysis.
EFFECT: method enables creating the instant model of anovulatory infertility close to physiological environment; it possesses good reproducibility of the results and a possibility to prolong the experiment period without damage to animal's life with no time limits.
1 tbl, 5 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF MODELLING OF POLYCYSTIC OVARIES | 2007 |
|
RU2337411C1 |
| APPLICATION OF TUNGSTEN (VI) SALTS FOR TREATMENT OF FEMALE INFERTILITY IN MAMMALS WITHOUT DIABETES | 2014 |
|
RU2635507C2 |
| METHOD OF REGULATION OF REPRODUCTIVE FUNCTION IN FEMALES | 1994 |
|
RU2076593C1 |
| METHOD FOR MODELLING SUPPRESSION OF OVARIAN HORMONAL ACTIVITY | 1999 |
|
RU2191584C2 |
| INSTANT DIAGNOSTIC TECHNIQUE FOR FEMALE ENDOCRINE INFERTILITY | 2014 |
|
RU2574669C2 |
| METHOD FOR SIMULATING CHRONIC ANOVULATION SYNDROME | 2013 |
|
RU2527166C1 |
| METHOD FOR MODELING POLYCYSTIC OVARIES AT PREDOMINANCE OF FOLLICULAR CYSTS | 2005 |
|
RU2282249C1 |
| AGENT FOR IMPROVING REPRODUCTIVE FUNCTION | 2012 |
|
RU2487705C1 |
| METHOD FOR MODELLING OF INBORN HYPERANDROGENITY | 0 |
|
SU1645989A1 |
| DIAGNOSTIC TECHNIQUE FOR REPRODUCTIVE OVARIAN POTENCY | 2008 |
|
RU2367949C1 |
