METHOD OF PRODUCING FOLLICULAR PROTEIN PREPARATION AND PREPARATION PRODUCED BY SAID METHOD Russian patent published in 2016 - IPC A61K35/00 

Abstract RU 2591819 C2

FIELD: biochemistry.

SUBSTANCE: method of producing follicular protein preparation comprises collecting follicular fluid of cattle, cooling down and after cooling centrifuging to obtain supernatant, mixing with cooled acetone, settling produced mixture, freeze-drying and cleaning by centrifugation to obtain freeze-dried product, which is dissolved in buffer, prepared suspension is centrifuged at 8,000-10,000 rpm for 15-30 minutes to produce supernatant, which is separated by ion-exchange chromatography on DEAE-Sepharose, collecting eluate coming at concentration of NaCl in an elution solution from 0.11 to 0.19 M, which is dialysed against water for not less than 8 hours to obtain preparation. Follicular protein preparation is obtained from follicular fluid of cattle. Preparation contains serum albumin and TGF-β protein, having immunospecificity of human inhibin βA, at following ratio of components, wt%: TGF-β protein, having immunospecificity of inhibin βA: 0.00005-3.0; serum albumin: balance.

EFFECT: group of inventions can be used for production of organic material of protein preparations, which can be used in medicine for reducing immunity in transplantology, treating endometriosis, certain oncological diseases, as well as in veterinary science.

2 cl

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RU 2 591 819 C2

Authors

Ilichev Aleksandr Vladimirovich

Maldov Dmitrij Grigorevich

Dates

2016-07-20Published

2014-03-03Filed