FIELD: biotechnology and biochemistry. SUBSTANCE: object of invention can find use as ingredient of therapeutic preparations, food additives, and element of diet therapy. Native fraction of chicken egg is isolated with distilled water, purified on water-equilibrated hydrophobic sorbent polychrome I. Active protein is eluted with distilled water after which inhibitor is subjected to rechromatography on ion-exchange sorbent. Molecular weight of inhibitor is 50120 Da and inhibitory activity with respect to trypsin 26.7 inh.un. EFFECT: increased yield.
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Authors
Dates
1999-04-27—Published
1997-12-31—Filed