METHOD OF PURIFICATION OF PROTEIN OF FAMILY OF GROWTH TRANSFORMING FACTORS PREPARED FROM FOLLICULAR FLUID Russian patent published in 2003 - IPC

FIELD: biological chemistry, proteins. SUBSTANCE: protein isolated from cattle ovary follicles is dissolved in buffer with neutral pH value and multiple-step and purification of obtained protein is carried out. Firstly, solution is subjected for filtration and gel-filtration and gel-filtration is carried out for the further two-stage purification of filtrate. At the first stage purification of filtrate is carried out by chromatography of high pressure by principle of reversed phase and at the second stage purification is carried out by preparative isoelectrofocusing. Invention provides the development of method of protein purification prepared from follicular fluid up to high purity degree being method excludes or minimizes the use of harmful substances and method can be realized under conditions of industrial manufacture with high percent of protein yield. Invention can be used for purification of protein of family of growth transforming factors (TGF-β). EFFECT: improved method of protein purification. 3 cl, 1 ex