FIELD: medicine.
SUBSTANCE: invention relates to molecular biology, virology, veterinary science and medicine and concerns method for identifying RNA of influenza viruses A and B with simultaneous determination of variants of hemagglutinin and neuraminidase of influenza virus A, as well as genetic markers of pathogenicity and resistance to influenza preparations on biological microchips. Method is based on reaction of reverse transcription to produce cDNA using viral RNA as matrix, polymerase chain reaction (PCR) and subsequent hybridization of obtained-fluorescent-marked PCR-product on biological microchip.
EFFECT: biological microchip is substrate with orderly arranged hydrogel elements including covalently immobilized oligonucleotide probes, providing hybridization with specific sections of influenza virus genome defining its type, subtype and genetic markers, including determinants of resistance to antiviral agents.
5 cl, 27 dwg, 4 tbl, 3 ex
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