FIELD: medicine.
SUBSTANCE: there are presented sets of oligonucleotide probes and primers, a biological microchip and a test system for identifying and typing influenza A and B virus. The characterised test system comprises: set of agents for recovering influenza virus RNA from human biological material; set of agents for performing PCR amplification combined with reverse transcription to form fluorescence labelled influenza virus genome containing the above oligonucleotide primers; set of agents for performing hybridisation of DNA fragments prepared after the amplification on the biochip comprising the elements with the immobilised described oligonucleotide probes; where it is necessary the negative and positive reference samples are provided.
EFFECT: presented inventions provide the more accessible and simple high-specificity and low-cost method.
4 cl, 2 dwg, 2 tbl, 4 ex
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Authors
Dates
2015-01-10—Published
2013-03-25—Filed