FIELD: biochemistry.
SUBSTANCE: invention relates to biochemistry, in particular to diagnostic technique for vegetable material on transgenicity. Method involves extraction of DNA from biomaterial and carrying out of polymerase chain reaction in real time with probes, marked by fluorescent dyes and fluorescence extinguishers. Wherein reaction mixture is made, containing primers aacacatgagcgaaacccta, caagctcgagtttctccata, ccggtcttgcgatgattat, tatattttgttttctatcgcgtatt, tatattttgttttctatcgcgtatt, agatggattgcacgcaggttc, gcatcagagcagccgattgt and probes FAM-cccttatctgggaactactcacacatta-BHQ1, FAM-tgcgggactctaatcataaaaacccatct-BHQ1, FAM-ccagtcatagccgaatagcctctccacc-BHQ1. Polymerase chain reaction is carried out in real time with continuous fluorescence control and its exponential rise shows transgenicity of plants.
EFFECT: invention allows effective diagnosis of plant material for transgenicity.
1 cl, 2 dwg, 1 tbl
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