METHOD FOR DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISM OF THE PRL-RSAI LOCUS USING SPECIFIC PRIMERS IN REAL-TIME POLYMERASE CHAIN REACTION Russian patent published in 2023 - IPC C12Q1/68 

FIELD: biotechnology; molecular genetics.

SUBSTANCE: method for detecting single nucleotide polymorphism of the PRL-RsaI locus using a test system has been proposed. The test system includes a mixture of deoxynucleotide triphosphates, a buffer for setting up the reaction, a thermostable enzyme - Taq polymerase, two synthetic oligonucleotide primers specific to a given DNA region: PRL-f CCTTATGAGCTTGATTCTTGGGTTGCTG; PRL-r ATATCATCTCCATGCCTTCCAGAAGTCG, two types of allele-specific fluorescent probes: PRLa - CGAGGTACGGGGTATG - FAM; PRLg - CGAGGTGCGGGGTATG - VIC, quenching probe common for both alleles: PRLbhq - (BHQ1) AAAGGAGCCCCAGATGCTAT - P, mineral oil that protects the reaction mixture from evaporation during PCR.

EFFECT: stable reproducibility of results when analyzing the number of DNA copies exceeding the sensitivity threshold of the test system.

1 cl, 1 dwg