FIELD: biochemistry.
SUBSTANCE: invention relates to biochemistry. Method of producing liquid sterile nutrient medium is disclosed. Method involves dissolving of dry components of nutrient medium in optimum volumes of solvent and sterilization of obtained solutions. Dissolution is performed for 5–10 minutes in ultrasonic bath with generator frequency of 37 kHz and amplitude of oscillations in range from 5 to 20 microns. Sterilizing ultrafiltration is carried out using cascade of three series-arranged membrane filters with pore size of 0.45–0.22–0.1 mcm and efficiency of filtration from 100 to 1,000 ml/min.
EFFECT: invention provides production of nutrient medium for cell biology in required volumes immediately before planned works, higher reliability and quality of operations with cells of mammals.
3 cl, 1 tbl, 1 dwg
