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2 307 166

(13)

(51)

IPC

C12Q1/22(2006-01-01)

C12M1/34(2006-01-01)

C12M1/36(2006-01-01)

C12N1/10(2006-01-01)

(21) (22)

Application

2005134698/13, 2005-11-10

(24)

Start date

2005-11-10

(22)

Actual filing date

2005-11-10

(45)

Published

2007-09-27

(72)

Inventor

Ruban Evgenij AleksandrovichDadasjan Artur JasharovichSamujlenko Anatolij Jakovlevich

(73)

Holder

Vserossijskij Nauchno-Issledovatel'Skij I Tekhnologicheskij Institut Biologicheskoj Promyshlennosti

METHOD FOR PROXIMATE DETERMINATION OF NON-STERILITY OF BROTH FOR MICROORGANISM, ANIMAL CELL AND VIRUS CULTIVATION IN BIOREACTORS Russian patent published in 2007 - IPC C12Q1/22 C12M1/34 C12M1/36 C12N1/10

Abstract RU 2307166 C2

FIELD: biotechnology, in particular biopreparation production.

SUBSTANCE: claimed method includes feeding of sterilized broth into presterilized inoculator or bioreactor equipped with means for redox-potential (eH) controlling, including eH electrode and microprocessor unit for controlling and adjustment of eH and pH measurement of redox potential value for 1 h under stirring and comparison of steady-state eH values with steady-state values. When redox-potential value deviates from steady-state value of broth redox-potential by 10 % said broth is recognized as non-sterile one.

EFFECT: process of decreased cost.

4 tbl, 4 ex

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RU 2 307 166 C2

Authors

Ruban Evgenij Aleksandrovich

Dadasjan Artur Jasharovich

Samujlenko Anatolij Jakovlevich

Dates

2007-09-27—Published

2005-11-10—Filed