FIELD: biotechnology. SUBSTANCE: method involves preparing a mixture containing dried inorganic salts, glucose and phenol red. Dried enzymatic hydrolyzate, prepared mixture and sodium hydrocarbonate are packaged into separate containers and components of medium are sterilized by accelerated electrons. Sterile components are dissolved in sterile purified water before using immediately. Invention provides preparing liquid sterile nutrient medium with high growth properties during all storage period for 2 years at temperature (6 ± 4) C. Prepared nutrient medium can be used for mammalian cells culturing. EFFECT: improved method of preparing, improved properties of medium. 4 cl, 3 tbl, 2 ex
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