FIELD: medicine, pharmacy.
SUBSTANCE: invention refers to experimental biology and medicine and provides a method of fluorescent histological detection of amyloid comprising fixation of organ tissue slices in 10% formaldehyde, staining by a fluorescent dye, water washing, dehydration, sealing into transparent non-fluorescent media, microscopy by means of a fluorescence microscope, using 4-N-aryl-3,5-dioxo-1-formyl-10-oxa-4-azatricyclo [5.2.11.7.02.6] Dec-8-enes 
formula where Y = 2-NO23-NO24-NO23-COOH 4-COOH derivatives as fluorescent dye, and dyeing is carried out by 1.5% alcoholic dye solution, mixed with 1% aqueous sodium hydroxide with a ratio of 1: 1.
EFFECT: invention provides increased information content due to the increased contrast of surface relief, and contributes to greater safety of histological sections.
3 tbl, 2 dwg, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF FLUORESCENT IDENTIFICATION OF AMYLOID | 2017 |
|
RU2673815C1 |
| METHOD FOR SIMULATING EXPERIMENTAL AMYLOID DISEASE IN ANIMALS | 2014 |
|
RU2572721C1 |
| METHOD FOR MODELING EXPERIMENTAL AMYLOIDOSIS IN ANIMALS | 2004 |
|
RU2269825C1 |
| PHARMACEUTICAL AGENT | 2006 |
|
RU2308270C1 |
| METHOD OF SIMULATING AMYLOIDOSIS IN ANIMALS | 2008 |
|
RU2373581C1 |
| METHOD OF PREVENTING SYSTEMIC AMYLOIDOSIS AND ITS NEPHROPATIC FORM IN EXPERIMENTAL ANIMALS | 2009 |
|
RU2410764C1 |
| METHOD FOR PROPHYLAXIS AND TREATMENT OF SYSTEMIC AMYLOIDOSIS AND NEPHROPATHIC FORM THEREOF IN EXPERIMENTAL ANIMALS | 2004 |
|
RU2270672C1 |
| METHOD FOR SIMULATION OF EXPERIMENTAL AMYLOIDOSIS IN ANIMALS | 2010 |
|
RU2446482C1 |
| EXOGENIC-INDUCED ANIMAL MODEL OF ALZHEIMER DISEASE | 2012 |
|
RU2532525C2 |
| BENZOTHIAZOLE DERIVATIVES, PHARMACEUTICAL COMPOSITION EXHIBITING PROPERTY TO BIND AMYLOID AND METHOD FOR DETECTING AMYLOID DEPOSITS IN MAMMAL | 2004 |
|
RU2440995C2 |
