METHOD OF PRODUCING HEPARIN Russian patent published in 2017 - IPC C08B37/10 

FIELD: chemistry.

SUBSTANCE: method of producing heparin involves thawing and homogenisation of raw material, filling with 0.6M sodium chloride solution, adding alcalase to a final concentration of 0.1-0.5%. Pigs' frozen mucous membrane of the small intestine (mucosa) with a shelf life of no more than 6 months at -20°C is used as raw material. Enzymatic hydrolysis is being performed at 65°C and pH 8.5-8.6 for 6 hours, then the enzyme is being inactivated at 90°C for 15 minutes. The produced hydrolyzate is cooled to temperature of 15-20°C and centrifuged. Concentrated hydrochloric acid is added to supernatant fluid to pH 3.0, heated to 70°C and being held for 30 minutes for additional hydrolyzate decontamination of protein origin ballast substances. The resulting residue is removed by way of centrifugation. Thereafter, end product sorption is performed in dynamic mode from the supernatant fluid on anion exchanger YMC-BioPro Q75. Anion exchanger is pre-equilibrated by 0.6M sodium chloride solution. After the sorption is complteted the anion exchanger is washed with 0.6M sodium chloride solution, ballast substances are removed from the anion exchanger with 0.9 M sodium chloride solution, heparin is desorbed from the anion exchanger with1.4 M sodium chloride solution.

EFFECT: invention increases output of heparin, its specific activity, reduces the technology process.

1 cl, 5 ex