FIELD: pharmaceuticals.
SUBSTANCE: invention relates to chemical-pharmaceutical industry and is a method for producing a complex of biologically active peptides from the prostate of slaughter animals, bull-calves or bulls, comprising the steps of grinding and homogenizing frozen tissue, extraction of homogenate with a solution of acetic acid, treatment of the supernatant with acetone, washing of the precipitate with acetone, drying precipitate, extracting peptides from the dried powder with water for injection, characterized in that extraction of the homogenate is carried out with a solution of 3 % acetic acid containing zinc chloride at a concentration of 1.2 g/l, at extraction stage of the peptides from the dried powder, the enzyme-serine endoprotease is added to a concentration of 0.3 %, maintained at a temperature of 65 °C with constant stirring for 3 hours, enzyme is inactivated at a temperature of 90 °C for 30 minutes, cooled to room temperature, separated or filtered and lyophilized.
EFFECT: invention allows to increase the yield and quality of the target product.
1 cl, 3 tbl, 3 ex
Title | Year | Author | Number |
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RU2292393C2 |
METHOD OF PREPARING SUBSTANCE SHOWING ANTIPROTEASE EFFECT | 1990 |
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SU1823184A1 |
METHOD OF PREPARING SUBSTANCE STIMULATING ANTIGEN-NONDEPENDING DIFFERENTIATION OF B-LYMPHOCYTES | 1999 |
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Authors
Dates
2018-10-15—Published
2017-08-14—Filed