METHOD FOR BACTERIAL LIPOPOLYSACCHARIDES RECOMBINANT PHOSPHATASE PRODUCING Russian patent published in 2017 - IPC C12N9/16 C12N1/21 C12N15/55 C12N15/63 C12N15/70 C12R1/19 

FIELD: biotechnology.

SUBSTANCE: method involves synthesis of a DNA sequence optimized for translation in E. coli, encoding LpxE F. novicida phosphatase protein. The said DNA sequence is shown in Fig.1A. This method provides subsequent construction of an expression vector encoding the chimeric precursor protein with N-terminal chimerization. The said precursor protein consists of a sequence of maltose-binding protein, protease factor Xa recognition site, six histidines, and LpxE F. novicida phosphatase. The method also involves obtaining a recombinant precursor protein production in E. coli, and its isolation and purification. The method comprises subsequent segregation of the maltose-binding protein from the polypeptide and final purification of the LpxE phosphatase protein.

EFFECT: invention allows to obtain recombinant phosphatase at a high yield.

6 dwg, 5 ex