FIELD: biotechnology.
SUBSTANCE: proposed enzymatically inactive IgA1 protease with replacement Ser267Ala for use as a component of a polyvalent vaccine designed to protect people against meningococcal infection and other microorganisms, which pathogenicity is caused by IgA1 protease. The invention includes a polynucleotide encoding the said mutant form of IgA1 protease of Neisseria meningitidis of serogroup B, comprising the said polynucleotide, a recombinant plasmid DNA, the strain Escherichia coli - producer of a mutant form of IgA1 protease according to the invention, the method of preparing a recombinant form of the enzyme using a technology of recombinant DNA, and recombinant inactivated IgA1 protease.
EFFECT: increased level of immunogenicity.
6 cl, 1 tbl, 5 dwg, 7 ex
| Title | Year | Author | Number |
|---|---|---|---|
| NUCLEIC ACID CODING FUNCTIONALLY ACTIVE RECOMBINANT IgAL PROTEASE NEISSERIA MENINGITIDIS OF SEROGROUP B, RECOMBINANT PLASMID DNA CONTAINING NUCLEOTIDE SEQUENCE CODING ACTIVE IgAL PROTEASE, PRODUCER STRAIN CONTAINING PLASMID DNA PRODUCING MATURE FORM OF IgAL PROTEASE, RECOMBINANT Ig PROTEASE NEISSERIA MENINGITIDIS OF SEROGROUP B, METHOD FOR PRODUCING MATURE FORM IgAL PROTEASE SHOWING IMMUNOGENIC AND PROTECTIVE PROPERTIES | 2011 |
|
RU2453599C1 |
| RECOMBINANT PROTEIN HAVING PROTECTIVE ACTION ON MENINGOCOCCUS (EMBODIMENTS), POLYNUCLEOTIDE CODING RECOMBINANT PROTEIN, RECOMBINANT PLASMID DNA CONTAINING SAID POLYNUCLEOTIDE, HOST CELL CONTAINING SAID RECOMBINANT PLASMID DNA, METHOD OF PRODUCING RECOMBINANT PROTEIN | 2017 |
|
RU2701964C2 |
| METHOD FOR PRODUCING IgAl-PROTEASE FROM SEROGROUP A NEISSERIA MENINGITIDIS AND RELATED IMMUNOGENIC PREPARATION | 2009 |
|
RU2407792C1 |
| METHOD FOR OBTAINING RECOMBINANT HUMAN OREXIN A , PLASMID DNA, STRAIN PRODUCER | 2016 |
|
RU2647771C2 |
| NEISSERIA MENINGITIDIS COMPOSITIONS AND METHODS OF USE THEREOF | 2013 |
|
RU2665841C2 |
| METHOD OF HIGH-LEVEL EXPRESSION, METHOD OF PURIFICATION AND METHOD OF OVER-STACKING OF PORINE PROTEIN OF MENINGOCOCCUS OF GROUP B EXTERNAL MEMBRANE OR PROTEIN FUSED WITH ITS, HIGHLY PURIFIED PORINE PROTEIN (VARIANTS), HIGHLY PURIFIED FUSED PROTEIN (VARIANTS), VACCINE FOR PROPHYLAXIS OF BACTERIAL MENINGITIDIS OF GROUP B, METHOD OF PREPARING CONJUGATE OF MENINGOCOCCUS OF GROUP B PROTEIN AND POLYSACCHARIDE, METHOD OF PROPHYLAXIS OF BACTERIAL MENINGITIDIS, STRAIN OF ESCHERICHIA COLI (VARIANTS) | 1994 |
|
RU2181378C2 |
| RECOMBINANT PLASMID DNA CODING FUSION PROTEIN L-HEP, ESCHERICHIA COLI STRAIN PRODUCER OF SAID PROTEIN AND METHOD OF PRODUCING RECOMBINANT PROTEIN | 2019 |
|
RU2716975C1 |
| RECOMBINANT PLASMID DNA OF PTRX-TEVRS-PTH CODING A HYBRID PROTEIN CAPABLE OF PROTEOLYTIC SPLITTING TO FORM A FRAGMENT OF ENDOGENOUS HUMAN PARATHYROID HORMONE (1-34), AN ESCHERICHIA COLI BL21(DE3)/PTRX-TEVRS-PTH STRAIN - PRODUCER OF SAID PROTEIN AND A METHOD OF PRODUCING RECOMBINANT PTH (1-34) | 2019 |
|
RU2700452C1 |
| VACCINE COMPOSITION CONTAINING TRANSFERRIN-BINDING PROTEIN AND HSF FROM GRAM-NEGATIVE BACTERIA | 2003 |
|
RU2359696C2 |
| RECOMBINANT PLASMID DNA FOR DETECTION OF AGENTS DAMAGING GENETIC APPARATUS OF CELL (VARIANTS) | 2005 |
|
RU2311459C2 |
