FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology. Disclosed is a recombinant protein having protective effect on meningococcus having amino acid sequence SEQ ID NO: 2, consisting of three fragments of primary structure of IgA protease Neisseria meningitidis serogroup B, connected in a single polypeptide chain. Also disclosed is a recombinant protein variant having the amino acid sequence SEQ ID NO: 4, consisting of two fragments of primary structure of IgA protease Neisseria meningitidis serogroup B, connected in a single polypeptide chain. Invention also discloses a polynucleotide encoding a corresponding recombinant protein, a recombinant plasmid DNA comprising a corresponding polynucleotide, a host cell transformed with recombinant plasmid DNA and producing a recombinant protein, and a method of producing a recombinant protein.
EFFECT: group of inventions provides producing highly purified immunogenic proteins with a high level of protective action on meningococci.
6 cl, 3 dwg, 2 tbl, 10 ex
| Title | Year | Author | Number |
|---|---|---|---|
| POLYNUCLEOTIDE ENCODING MUTANT RECOMBINANT IgA1 PROTEASE OF Neisseria meningitidis OF SEROGROUP B, RECOMBINANT PLASMID DNA COMPRISING SAID POLYNUCLEOTIDE, HOST CELL CONTAINING SAID PLASMID DNA, RECOMBINANT IgA1 PROTEASE OF Neisseria memingitidis OF SEROGROUP B, METHOD OF PRODUCING MATURE FORM OF IgA1 PROTEASE | 2011 |
|
RU2486243C1 |
| NUCLEIC ACID CODING FUNCTIONALLY ACTIVE RECOMBINANT IgAL PROTEASE NEISSERIA MENINGITIDIS OF SEROGROUP B, RECOMBINANT PLASMID DNA CONTAINING NUCLEOTIDE SEQUENCE CODING ACTIVE IgAL PROTEASE, PRODUCER STRAIN CONTAINING PLASMID DNA PRODUCING MATURE FORM OF IgAL PROTEASE, RECOMBINANT Ig PROTEASE NEISSERIA MENINGITIDIS OF SEROGROUP B, METHOD FOR PRODUCING MATURE FORM IgAL PROTEASE SHOWING IMMUNOGENIC AND PROTECTIVE PROPERTIES | 2011 |
|
RU2453599C1 |
| RECOMBINANT PLASMID DNA CODING FUSION PROTEIN L-HEP, ESCHERICHIA COLI STRAIN PRODUCER OF SAID PROTEIN AND METHOD OF PRODUCING RECOMBINANT PROTEIN | 2019 |
|
RU2716975C1 |
| RECOMBINANT PLASMID DNA OF PTRX-TEVRS-PTH CODING A HYBRID PROTEIN CAPABLE OF PROTEOLYTIC SPLITTING TO FORM A FRAGMENT OF ENDOGENOUS HUMAN PARATHYROID HORMONE (1-34), AN ESCHERICHIA COLI BL21(DE3)/PTRX-TEVRS-PTH STRAIN - PRODUCER OF SAID PROTEIN AND A METHOD OF PRODUCING RECOMBINANT PTH (1-34) | 2019 |
|
RU2700452C1 |
| METHOD FOR OBTAINING RECOMBINANT HUMAN OREXIN A , PLASMID DNA, STRAIN PRODUCER | 2016 |
|
RU2647771C2 |
| RECOMBINANT PLASMID DNA ENCODING HYBRID POLYPEPTIDES WITH PROPERTIES OF RED FLUORESCENT PROTEIN mCherry TO PRODUCE HYBRID FLUORESCENT PROTEINS IN Escherichia coli | 2013 |
|
RU2527171C1 |
| FUSED PROTEIN OF THIOREDOXIN AND INFESTIN 4 DOMAIN, METHOD FOR PREPARING IT, EXPRESSION PLASMID DNA CODING FUSED PROTEIN, AND BACTERIUM Escherichia coli TRANSFORMED BY THIS PLASMID DNA | 2012 |
|
RU2528251C2 |
| RECOMBINANT PLASMID DNA pEFN877, DETERMINING EXPRESSION OF THE HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE SURFACE OF ESCHERICHIA COLI CELLS, AND STRAIN OF BACTERIA ESCHERICHIA coli BL21(DE3)pLysS/pEFN877 - PRODUCER OF HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE CELLS SURFACE | 2015 |
|
RU2606014C1 |
| RECOMBINANT PLASMID DNA PSMT3_HCRG21 ENCODING A HYBRID PROTEIN SMT3-HCRG21, BACTERIAL STRAIN ESCHERICHIA COLI BL21(DE3)/PSMT3_HCRG21 - PRODUCER OF HCRG21 ANALGESIC PEPTIDE AND METHOD OF PRODUCING RECOMBINANT HCRG21 ANALGESIC PEPTIDE | 2022 |
|
RU2798545C1 |
| RECOMBINANT PLASMID DNA PET3.54, ENCODING POLYPEPTIDE FN3.54, INTERACTING WITH HUMAN TUMOUR NECROSIS FACTOR, AND STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF POLYPEPTIDE FN3.54, INTERACTING WITH HUMAN TUMOUR NECROSIS FACTOR | 2013 |
|
RU2531547C1 |
