FIELD: biotechnology.
SUBSTANCE: method for obtaining of a combined enzyme beta-galactosidases preparation involves preparation of lactose-containing raw material with lactose mass fraction of 3-15%. Lactose-fermenting yeast and thermophilic lactic acid bacteria are introduced into the lactose-fermenting raw material and cultivated at a temperature of (30±2)°C for 7-16 hours, followed by enzymes extraction by autolysis at a temperature of (50±5)°C for 6-12 hours, enzymes purification by ultrafiltration and concentration to obtain a combined enzyme preparation.
EFFECT: invention allows to simplify method for enzyme preparation obtaining.
2 ex
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