FIELD: biotechnology.
SUBSTANCE: method involves implantation of a microfluidic device that contains a cell growth chamber, an open port, as well as a demineralized bone powder and bone morphogenetic protein, into the subject for 4 weeks or more, resulting in bone marrow tissue formation. Thereafter, the said microfluidic device with the formed tissue contained therein is recovered from the subject. Further, liquid perfusion to the bone marrow tissue is provided to maintain the bone marrow tissue ex vivo. Microfluidic device implantation may be subcutaneous, intraperitoneal or intramuscular.
EFFECT: invention allows to obtain bone marrow tissue that will preserve ex vivo functioning.
8 cl, 21 dwg, 9 ex
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