FIELD: biotechnology.
SUBSTANCE: method for determination of mycobacteria tuberculosis resistance to antitubercular preparations of the second series - fluoroquinolones, aminoglycosides and capreomicin, sets of detection probes and amplification primers and method for confirmation of the clinical diagnosis of extensively drug-resistant tuberculosis. The method involves simultaneous detection of mutations in the tuberculosis mycobacteria DNA in a biological sample, leading to microorganisms resistance to antitubercular preparation of the second series in the sites of gyrA gene (codons 89, 90, 91, 94), gyrB gene (538, 539, 540), rrs gene (positions 1401, 514, 517) and the eis promoter region (positions -10, -12, -37), by real-time MPCR using primers for amplification and detection probes complementary to these gene sites.
EFFECT: simultaneous and rapid determination of tuberculosis mycobacteriua resistance to second-series preparations, and can be used in the diagnosis of extensively drug-resistant tuberculosis.
11 cl, 5 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| DETECTION METHOD OF DNA OF TUBERCULOSIS CAUSATIVE AGENT WITH SIMULTANEOUS IDENTIFICATION OF ITS GENOTYPE AND DETERMINATION OF GENETIC DETERMINANTS OF MULTIPLE AND WIDE DRUG RESISTANCE, OLIGONUCLEOTIDE MICROCHIP, SET OF PRIMERS AND SET OF OLIGONUCLEOTIDE PROBES, WHICH ARE USED IN METHOD | 2014 |
|
RU2562866C1 |
| DIAGNOSTICS METHOD OF SENSITIVITY OF Mycobacterium tuberculosis STRAINS TO INJECTION ANTITUBERCULOUS PREPARATIONS OF RESERVE ROW (AMINOGLYCOSIDES AND CAPREOMYCIN) | 2012 |
|
RU2509158C2 |
| PRIMERS AND PROBES FOR POLYMERASE CHAIN REACTION FOR DETECTING MYCOBACTERIUM TUBERCULOSIS | 2015 |
|
RU2717655C2 |
| DIAGNOSTIC TECHNIQUE FOR OF MYCOBACTERIUM TUBERCULOSIS STRAIN SENSITIVITY TO AMINOGLYCOSIDES | 2009 |
|
RU2409680C1 |
| METHOD FOR ESTIMATING ANTIMYCOBACTERIAL ACTION OF ANTI-TUBERCULOSIS DRUGS USING BIOLOGICAL MATERIAL OF PATIENTS WITH PULMONARY TUBERCULOSIS | 2017 |
|
RU2677972C1 |
| METHOD OF DIAGNOSING SENSITIVITY OF Mycobacterium tuberculosis STRAINS TO FLUOROCHINOLONES BY GENE gyr B | 2010 |
|
RU2439162C1 |
| METHOD OF DIAGNOSTICS OF SENSITIVITY OF MYCOBACTERIUM TUBERCULOSIS STRAINS TO FLUOROQUINOLONES | 2004 |
|
RU2343197C2 |
| METHOD FOR DETERMINATION OF MYCOBACTERIUM TUBERCULOSIS RESISTANCE TO RIFAMPICIN AND ISONIAZID | 2015 |
|
RU2619258C2 |
| STRAIN MYCOBACTERIUM TUBERCULOSIS 326/18/47 B-9673 FOR EVALUATION OF THE EFFECTIVENESS OF ANTI-TB DRUGS AND DISINFECTANTS | 2022 |
|
RU2785409C1 |
| METHOD OF SIMULTANEOUS AMPLIFICATION AND FLUORESCENT MARKING OF SEVERAL GENOME SEGMENTS OF MYCOBACTERIA OF TUBERCULOSIS COMPLEX | 2013 |
|
RU2548797C2 |
