FIELD: medicine.
SUBSTANCE: group of inventions is a method for determination of mycobacterium tuberculosis resistance to rifampicin and isoniazid in a biological sample, wherein the said method includes simultaneous detection of mutations in the bacterial DNA of the biological sample in rpoB gene, katG gene portions and inhA gene promoter region, wherein the said sections are selected from the group consisting of codons 430, 445, 450 and 452 of rpoB gene, codon 315 of katG gene, position -15 or -17 of the promoter region of inhA gene or a combination thereof, by multiplex polymerase chain reaction in real time using detection probes that are complementary to these portions of genes containing no mutation. The group of inventions also refers to application of this method to confirm the clinical diagnosis of multidrug resistance, and a set of probes to implement the said method.
EFFECT: simultaneous and rapid determination of the Mycobacterium tuberculosis resistance to rifampicin and isoniazid with high detectability.
7 cl, 2 ex, 3 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| DETECTION METHOD OF DNA OF TUBERCULOSIS CAUSATIVE AGENT WITH SIMULTANEOUS IDENTIFICATION OF ITS GENOTYPE AND DETERMINATION OF GENETIC DETERMINANTS OF MULTIPLE AND WIDE DRUG RESISTANCE, OLIGONUCLEOTIDE MICROCHIP, SET OF PRIMERS AND SET OF OLIGONUCLEOTIDE PROBES, WHICH ARE USED IN METHOD | 2014 |
|
RU2562866C1 |
| METHOD OF SIMULTANEOUS AMPLIFICATION AND FLUORESCENT MARKING OF SEVERAL GENOME SEGMENTS OF MYCOBACTERIA OF TUBERCULOSIS COMPLEX | 2013 |
|
RU2548797C2 |
| METHOD FOR MOLECULAR-GENETIC DETECTION OF TUBERCULOSIS MICROBACTERIA RESISTANCE TO ANTITUBERCULAR PREPARATIONS OF SECOND SERIES (FLUOROQUINOLONES, AMINOGLYCOSIDES AND CAPREOMICIN) | 2015 |
|
RU2633507C2 |
| METHOD OF TREATING INFILTRATIVE PULMONARY TUBERCULOSIS INDUCED BY MULTIPLE-DRUG-RESISTANT AND HIGHLY TOXIC STRAINS OF MYCOBACTERIA TUBERCULOSIS | 2009 |
|
RU2423129C1 |
| METHOD OF EXPRESS DETERMINATION OF ISONIAZIDUM TOLERANCE AT TUBERCULOSIS MICOBACTERIA | 2006 |
|
RU2339040C2 |
| STRAIN MYCOBACTERIUM TUBERCULOSIS 326/18/47 B-9673 FOR EVALUATION OF THE EFFECTIVENESS OF ANTI-TB DRUGS AND DISINFECTANTS | 2022 |
|
RU2785409C1 |
| DIAGNOSIS OF MYCOBACTERIUM TUBERCULOSIS STRAIN SENSITIVITY TO ISONIASIDE | 2003 |
|
RU2297456C2 |
| METHOD FOR SIMULTANEOUS DETECTION OF MYCOBACTERIA OF TUBERCULOSIS COMPLEX AND IDENTIFICATION OF MUTATIONS IN DNA OF MYCOBACTERIA, WHICH RESULT IN MICROORGANISMS RESISTANCE TO RIFAMPICIN AND ISONIAZID, ON BIOLOGICAL MICROCHIPS, SET OF PRIMERS, BIOCHIP AND SET OF OLIGONUCLEOTIDE PROBES USED IN METHOD | 2005 |
|
RU2376387C2 |
| METHOD FOR QUICK TEST DETECTION ON RESISTANCE TO RIFAMPICIN IN TUBERCULOSIS MYCOBACTERIA | 2003 |
|
RU2287586C2 |
| METHOD OF DETECTION OF Mycobacterium tuberculosis ISOLATES RESISTANT TO PYRAZINAMIDE | 2013 |
|
RU2552214C2 |
