FIELD: veterinary medicine; microbiology.
SUBSTANCE: invention refers to veterinary microbiology. Method of preparing the nutrient medium for growing probiotic cultures involves the production of soy hydrolyzate at the rate of 24-48 g of dry soy milk per 1 liter of water by means of hydrolysis with pepsin for 48 hours in order to obtain the hydrolyzate. Resulting hydrolyzate is subjected to filtration and sterilization, followed by the addition of peptone, glucose and water in the predetermined ratio of components in order to produce the nutrient medium. Nutrient medium is heated, cooled, filtered, the pH of the medium is set within the range of 6.8-7.0 and they are sterilized at the specified parameters.
EFFECT: invention allows to simplify the method of preparing the nutrient medium.
1 cl, 4 ex
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Authors
Dates
2018-03-01—Published
2016-10-14—Filed