FIELD: biochemistry; biotechnology.
SUBSTANCE: invention relates to the field of biochemistry and biotechnology, in particular to recombinant plasmid DNA pET15/N-Dest+. Plasmid DNA is intended for expression in the Escherichia coli cells of the destabilase gene of the medicinal leech Hirudo medicinalis. Invention also concerns a method of producing plasmid DNA pET15/N-Dest+ and to the strain Escherichia coli BL21(DE3)Gold/pET15/N-Dest+, obtained by transformation of the strain Escherichia coli BL21(DE3)Gold of plasmid DNA pET15/N-Dest+. Strain is a producer of recombinant destabilase. Invention also relates to a method of producing strain Escherichia coli BL21(DE3)Gold/pET15/N-Dest+ and a method for producing recombinant destabilase using the above strain.
EFFECT: invention makes it possible to obtain recombinant destabilase with high yield and purity.
10 cl, 4 dwg, 2 tbl, 6 ex
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