FIELD: biotechnology, genetic protein engineering. SUBSTANCE: method is based on the use of new recombinant plasmid DNA p3PTEIL3 where an artificial gene of the human interleukin-3 is under control of three promoters tandem (P8, Ptac and Pt7) and its expression is carried out after induction with isopropylthiogalactoside and the use of the strain-producer E. coli VKPM B-6968 that provides high level of biosynthesis of the end protein (up to 30% of total cellular protein). Recombinant plasmid has tandem of promoters P8, Ptac and Pt7, two lac-operators, enhancer of translation of gene-10 of phage T7, terminator of transcription of phage fd, gene of β-lactamase and an artificial gene of the human interleukin-3. Strain Escherichia coli VKPM B-6968 is obtained by transformation of competent cells E. coli BL21(DE3) ekfpvbljq p3PTEIL3. Strain E. coli VKPM B-6968 is cultured on rich medium, the grown cells were disrupted in buffer solution and the human recombinant interleukin-3 is isolated from inclusion bodies followed by its renaturation by the known method. EFFECT: improved method of preparing. 3 cl, 3 dwg
