FIELD: biotechnology.
SUBSTANCE: invention relates to the area of biotechnology and genetic engineering. A recombinant plasmid DNA pACYC-LANS(KM) was constructed to be expressed in the cells of Escherichia coli of the polypeptide of recombinant L-asparaginase of Erwinia carotovora (rec-ASP-ECAR), was proposed producer strain rec-ASP-ECAR that is obtained from transformation of competent cells of Escherichia coli BL21(DE3) using the constructed recombinant plasmid DNA pACYC-LANS(KM), a method for growing the strain with its splitting from the obtained biomass and purification of recombinant L-asparaginase from Erwinia carotovora was developed.
EFFECT: enhanced biosynthesis of rec-ASP-ECAR polypeptide and high output and purity of the target product which is achieved by the implementation of a simple method for producing recombinant asparaginase.
4 cl, 4 dwg, 2 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PRODUCING A SUBSTANCE OF THE RECOMBINANT L-ASPARAGINASE FROM ERWINIA CAROTOVORA | 2010 |
|
RU2441914C1 |
| GENE ENCODING L-ASPARAGINASE IN ERWINIA CAROTOVORA AND STRAIN ESCHERICHIA COLI VKPM = B-8174 AS PRODUCER OF ERWINIA CARATOVORA L-ASPARAGINASE | 2001 |
|
RU2221868C2 |
| MODIFIED RAS GENE OF ESCHERICHIA COLI BACTERIUM, CODING PRECURSOR OF ENZYME WITH PENICILLIN G ACYLASE ACTIVITY, RECOMBINANT ESCHERICHIA COLI STRAIN - PRODUCER OF ACYLASE PENICILLIN G AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF THIS ENZYME | 2015 |
|
RU2624022C1 |
| RECOMBINANT PLASMID DNA pET15/N-Dest+ AND METHOD FOR THE PREPARATION, THE STRAIN OF ESCHERICHIA COLI BL21(DE3)GOLD, TRANSFORMED BY RECOMBINANT DNA pET15/N-Dest+, AND A METHOD OF ITS RECEPTION, A METHOD FOR OBTAINING RECOMBINANT BIFUNCTION ENZYME - DESTABILASE OF MEDICAL LEECH | 2015 |
|
RU2658429C2 |
| STRAIN Escherichia coli BL21(DE3)Gold/pETmin-CypA PRODUCING HUMAN RECOMBINANT CYCLOPHILIN A | 2013 |
|
RU2557305C2 |
| RECOMBINANT PLASMID DNA PACYCLANS FOR TRANSFER AND EXPRESSION GENE ENCODING ERWINIA CAROTOVORA (ECAR-LANS) L-ASPARAGINASE IN ESCHERICHIA COLI CELLS AND METHOD FOR PREPARING RECOMBINANT ECAR-LANS FROM BIOMASS OF ESCHERICHIA COLI PRODUCER STRAINS | 2002 |
|
RU2224797C2 |
| RECOMBINANT PLASMID DNA ENCODING HYBRID POLYPEPTIDES WITH PROPERTIES OF RED FLUORESCENT PROTEIN mCherry TO PRODUCE HYBRID FLUORESCENT PROTEINS IN Escherichia coli | 2013 |
|
RU2527171C1 |
| RECOMBINANT PLASMID pSVH0106 PROVIDING SYNTHESIS OF Gl7ACA ACYLASE IN Escherichia coli CELLS, RECOMBINANT STRAIN OF Escherichia coli BL21(DE3)/pSVH0106 AS PRODUCER OF Gl7ACA ACYLASE | 2005 |
|
RU2300566C2 |
| RECOMBINANT PLASMID DNA PTLS CONTAINING GENE OF SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS AND STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF SINGLE-STRANDED ANTIBODIES AGAINST TICK-BORNE ENCEPHALITIS VIRUS | 1998 |
|
RU2158309C2 |
| RECOMBINANT PLASMID DNA PSS5 ENCODING SYNTHESIS OF RECOMBINANT HUMAN ALPHA-2B INTERFERON, STRAIN OF ESCHERICHIA COLI SS5 AS PRODUCER OF RECOMBINANT HUMAN ALPHA-2B INTERFERON AND METHOD OF ALPHA-2B INTERFERON PREPARING | 1999 |
|
RU2165455C1 |
