METHOD FOR DETERMINING THE INFLUENCE OF LOW-MOLECULAR BIOLOGICALLY ACTIVE SUBSTANCES ON THE AFFINITE OF PROTEIN-LIGAND BONDING Russian patent published in 2019 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to clinical chemistry, and can be used to identify the effect of low-molecular biologically active substances on the affinity of the protein-ligand bonding. For this purpose, quantitative determination of the titer of hepatitis antigens of viral etiology in the serum of patients by enzyme multiplied immunoassay is carried out. In this case, the biologically active compound of low molecular weight is introduced into a well of plate with immobilized antibodies to the hepatitis virus antigen before the applying of serum to determine the effect on antibodies or antigens. After that, incubate for 5 minutes in a dark place and conduct an enzyme multiplied immunoassay. Build a calibration graph of the dependence of optical density on concentration of viral hepatitis antigen. According to the obtained calibration graph, the titer of antigens in control and experimental samples is calculated. When changing antigen concentration by ≥±10 % compared to a control sample to draw conclusions about the presence of influence of the investigated low-molecular biologically active substances on the affinity of protein-legend bonding. And when changing the antigen concentration by <±10 % – about the absence of the influence of the selected low-molecular biologically active substance on the affinity of protein-ligand bonding.

EFFECT: invention allows to identify the effect of low molecular weight biologically active substances on the interaction of proteins with ligands by determining the titer of hepatitis antigens of viral etiology in the serum of patients.

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