METHOD OF THE DIFFERENTIAL DIAGNOSIS OF ACUTE LEUKEMIA USING A CELLULAR BIOCHIP Russian patent published in 2019 - IPC G01N33/50 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, in particular, to the diagnosis of oncohematological diseases, and can be used for the differential diagnosis of acute leukemia. Method involves sorting an isolated mononuclear fraction of leukocytes by their surface antigens by incubating on three cell biochips with antibodies immobilized on a transparent substrate. Then the biochips are washed from unbound leukocytes and dried, after which biochip 1 is stained for morphological examination, biochip 2 is used to determine intracellular markers, and biochip 3 is used for the analysis of cytochemical activity. Presence of acute leukemia is confirmed, if in the morphological analysis of leukocytes bound to anti-CD45RA on biochip 1, the proportion of blast cells among them exceeds 25 %, in this case, based on the presence of binding of blast cells with antibodies to a set of CD antigens characteristic of myeloid, monocytic and lymphoid cells, the myelomonocytic MM score is determined by the formula MM=p(CD13)+p(CD33)+p(CD117)+p(CD14)+p(CD64)+0.5*p(CD11b)+0.5*p(CD15) and the B-lymphocyte score B is determined by the formula B=p(CD10)+p(CD19)+(CD22), where in the presence of binding of blast cells with antibody to CDi p(CDi)=1, and in the absence of binding of blast cells with antibody to CDi p(CDi)=0. Moreover, if myelomonocytic score is <2, and B-lymphocyte score is ≥2, diagnosis of B lymphoblastic leukemia (B-ALL) is established, if the myelomonocytic score is ≥2, acute myeloblastic leukemia (AML) M2, M3, M4 or M5 is diagnosed, if myelomonocytic score is <2 and B-lymphocytic score is <2, acute myeloblastic leukemia M0, M1, M7 is diagnosed or T-lymphoblastic leukemia (T-ALL), while if blast cells are present on the antibodies to CD41 and/or CD61, acute myeloblastic leukemia, M7, is diagnosed, and in the presence of intracellular CD3 in blast cells, T-ALL is diagnosed.

EFFECT: use of the invention allows to improve the accuracy of the differential diagnosis of AML and ALL.

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