FIELD: medicine.
SUBSTANCE: method of study of cells by the means of immunological biochip is proposed, which contains capture antibodies, specific to cell membrane antigens. Biochip is incubated with cell suspension, and then it is washed out from cells not linked to the antibodies. Then the biochip is treated with a solution which increases the strength of binding the biochip and cells remaining on its surface due to interaction with immobilised antibodies. Preparation for microscopic studies is prepared of the biochip, research study of related to the biochip cells is conducted, reading and analysing the result is performed. In the course of the study colouring of connected cells can be done or their treatment with labeled antibodies can be conducted.
EFFECT: method enables to prevent cells detachment from the surface of a biochip at conducting of different manipulations, which leads to increased sensitivity of analysis.
13 cl, 6 dwg, 2 ex
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Authors
Dates
2010-05-10—Published
2008-12-02—Filed