FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, specifically to recombinant production of therapeutic proteins, and can be used to produce recombinant anti-tumour protein DR5-B in E. coli. Method involves transformation of cells of the E. coli SHuffle strain B in the produced recombinant plasmid DNA pET-32a without Trx-, His- and S-tags coding DR5-B with subsequent expression, extraction and purification of the target protein.
EFFECT: invention enables to obtain a modified anti-tumour protein Manselan based on a TRAIL DR5-B mutant variant of cytokine with high output and degree of purification from low-molecular protein impurities and bacterial endotoxins.
1 cl, 2 tbl, 6 dwg, 4 ex
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