RECOMBINANT PLASMID DNA OF PTRX-TEVRS-PTH CODING A HYBRID PROTEIN CAPABLE OF PROTEOLYTIC SPLITTING TO FORM A FRAGMENT OF ENDOGENOUS HUMAN PARATHYROID HORMONE (1-34), AN ESCHERICHIA COLI BL21(DE3)/PTRX-TEVRS-PTH STRAIN - PRODUCER OF SAID PROTEIN AND A METHOD OF PRODUCING RECOMBINANT PTH (1-34) Russian patent published in 2019 - IPC C07K14/635 C07K19/00 C12N15/16 C12N15/62 C12N15/70 C12N1/21 C12P21/02 C12R1/19 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, specifically to recombinant production of therapeutic proteins and can be used to produce active fragment (1-34) of endogenous human parathyroid hormone. Recombinant plasmid DNA pTrx-TEVrs-PTH, which provides synthesis of a hybrid protein containing a sequence of thioredoxin and teriparatide in Escherichia coli cells, is constructed. By transforming strain E.coli BL 21(DE3) with plasmid pTrx-TEVrs-PTH, a producer strain of Trx-TEVrs-PTH hybrid protein is obtained. A method for producing recombinant teriparatide providing for production and cultivation of a producer of a hybrid protein containing teriparatide with subsequent extraction and splitting of said hybrid protein.

EFFECT: invention enables to obtain recombinant teriparatide with high output and simplified technology.

7 cl, 4 dwg, 3 ex