FIELD: chemistry.
SUBSTANCE: group of inventions relates to a recombinant yeast host cell for xylitol production and use thereof. Said host cell produces D-arabit from glucose and contains a heterologous nucleic acid sequence encoding NAD+-specific D-arabit-4-oxidoreductase (EC 1.1.1.11), using D-arabitol as a substrate and giving D-xylulose as a product, and a heterologous nucleic acid sequence encoding NADPH-specific xylitol dehydrogenase using D-xylulose as a substrate and producing xylitol as a product. What is also presented is a method for producing xylitol using said host cell and using said host cell to produce xylitol.
EFFECT: group of inventions provides higher production of xylitol.
16 cl, 47 dwg, 16 tbl, 27 ex
| Title | Year | Author | Number |
|---|---|---|---|
| XYLOSE ISOMERASE-EXPRESSING MICROORGANISM | 2009 |
|
RU2553537C2 |
| METHOD OF XYLITOL PRODUCING | 1993 |
|
RU2142999C1 |
| METHOD OF PREPARING GENETICALLY MODIFIED BACTERIA SORANGIUM/POLYANGIUM AND RECOMBINANT PLASMID DNA MOLECULE | 1992 |
|
RU2124053C1 |
| POLYPEPTIDES FOR ENANTIOSELECTIVE ENZYMATIC RESTORATION OF INTERMEDIATE COMPOUNDS | 2008 |
|
RU2486239C2 |
| ENZYMATIC PRODUCTION OF 1-BUTANOL | 2006 |
|
RU2429295C2 |
| PRODUCT AND METHOD | 2006 |
|
RU2385931C1 |
| METHOD OF PREPARATION OF RECOMBINANT BIRDS VIRUS FOR VACCINATION AND GENETIC THERAPY | 2007 |
|
RU2326942C1 |
| RECOMBINANT PLASMID DNA pMALTEV-legumain, ENCODING POLYPEPTIDE HAVING ANTIGENIC PROPERTIES OF PROTEIN LEGUMAIN Opisthorchis felineus, AND STRAIN E.coli BL 21(DE3)pLysS-pMALTEV-legumain - PRODUCENT OF RECOMBINANT POLYPEPTIDE HAVING ANTIGENIC PROPERTIES OF PROTEIN LEGUMAIN Opisthorchis felineus | 2012 |
|
RU2496876C1 |
| GENES ENCODING MORAXELLA TRANSFERRIN RECEPTOR | 1997 |
|
RU2235128C2 |
| PRODUCTION OF GLYCOPROTEINS WITH MODIFIED FUCOSYLATION | 2008 |
|
RU2479629C2 |
