FIELD: medicine.
SUBSTANCE: invention refers to oncology and pharmacy, and can be used to determine the minimum effective concentration of an antitumour drug which inhibits cytoprotective autophagy. That is ensured by exposing the human tumour cells to culture for 24 hours. Cells are then stained with two fluorescent dyes of acridine orange and propidium iodide. Percentage of living cells is counted with autophagosomes in relation to all living cells. Minimally effective concentration of the drug preparation is determined, wherein the percentage of living cells with autophagosomes in relation to all living cells will be equal to zero.
EFFECT: invention provides higher clinical effectiveness in malignant new growths by determining minimum effective concentrations of anticancer drugs.
1 cl, 3 dwg, 1 tbl, 1 ex
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