TEST SYSTEM FOR DETECTING RNA OF SCHMALLENBERG DISEASE VIRUS IN FARM ANIMALS Russian patent published in 2019 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: to increase diagnostic accuracy, a test system for detecting RNA of Schmallenberg disease virus in farm animals includes a buffer for carrying out a polymerase chain reaction, mixture for its carrying consisting of deoxynucleoside triphosphates, primers and fluorescent probes specific for agent of Schmallenberg disease virus and for internal control sample; mixture of enzymes from DNA polymerase with antibodies inhibiting enzyme activity, TAQ POLYMERASE; RNA dilution buffer, internal control sample, negative control sample, positive control sample, according to invention for internal control sample suspension of bacteriophage MS2 with concentration of 5×10³ copies of nucleotide sequences per 1 mcl, and for a positive control sample, a mixture of recombinant plasmid DNA containing a fragment of the genome of the Schmallenberg virus exciter and a fragment of the bacteriophage MS2 genome, taken in ratio 1:1 with the following nucleotide sequences: Shm-F 5' TCAGATTGTCATGCCCCTTGC 3'` – forward primer; Shm-R 5' TCGGCCCCAGGTGCAAATC 3' – reverse primer; Shm-Z JOE – ACCATCGGCCCAGGTGCATCCCTTA-BHQ1 – probe; MS2F, 5'-TGGCACTACCCCTCTCCGTATTCAC-3' – forward primer; MS2R, 5'-GTACGGGCGACCCCACGATGAC-3' – reverse primer; MS2P, 5'-Cy5-CACATCGATAGATCAAGGTGCCTACAAGC-3' BHQ2 – probe.

EFFECT: invention provides more accurate diagnosis of Schmallenberg disease in farm animals.

1 cl, 3 dwg, 4 tbl