TEST SYSTEM FOR DETECTING CORONAVIRUS INFECTION PATHOGEN GENOME IN CATTLE BY MEANS OF MULTIPLEX POLYMERASE CHAIN REACTION WITH FLUORESCENT DETECTION IN REAL TIME Russian patent published in 2019 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: described is a test system for detecting the coronavirus infection pathogen genome in cattle using a multiplex polymerase chain reaction (PCR) with real-time fluorescent detection. Test system comprises a PCR buffer, a mixture for its carrying out, consisting of deoxynucleoside triphosphates, primers and fluorescent probes specific for coronavirus A and for an internal control sample; mixture of enzymes from DNA polymerase with antibodies inhibiting enzyme activity, TAQ POLYMERASE and reverse transcriptase MMLV REVERSE TRANSCRIPTASE. Mixture of recombinant plasmid DNA containing a fragment of BCoV virus genome and a fragment of bacteriophage MS2 genome contains the following nucleotide sequences: BCoVF 5'-GATCAAATTGCTAGT-3' - forward primer; BCoVR 5'-CAGTCTGCTTAGTTA-3' - reverse primer; BCoVP 5'-FAM-GGATGCCACTAAGCCA-3' - BHQ1 - probe; MS2F 5'-TGGCACTACCCCTCTCCGTATTCAC-3' - forward primer; MS2R, 5'-GTACGGGCGACCCCACGATGAC-3' - reverse primer; MS2P Cy5 5'-CACATCGATAGATCAAGGTGCCTACAAGC-3 BHQ' is a probe.

EFFECT: invention extends the range of means for detecting coronavirus infection genome in cattle.

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