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2 696 069

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IPC

C12Q1/00(2006-01-01)

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Application

2018101633, 2018-01-16

(24)

Start date

2018-01-16

(22)

Actual filing date

2018-01-16

(45)

Published

2019-07-30

(72)

Inventor

Chernykh Oleg YurevichSochnev Vasilij VasilevichBannov Vasilij AleksandrovichMalyshev Denis VladislavovichDrobin Yurij DmitrievichDonnik Irina MikhajlovnaIvanov Arkadij VasilevichLysenko Aleksandr AnatolevichKrivonos Roman AnatolevichShevkoplyas Vladimir NikolaevichKoshchaev Andrej GeorgievichKaloshkina Inna MuratovnaKulakova Anna Leonidovna

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniya Gosudarstvennyj Agrarnyj Universitet Imeni I.T. Trubilina"

METHOD FOR DETECTING GENOME OF PARAINFLUENZA VIRUS OF TYPE 3 IN BOVINE ANIMALS Russian patent published in 2019 - IPC C12Q1/00

Abstract RU 2696069 C2

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry. Described is a method for detecting a genome of parainfluenza virus of type 3 in cattle, involving extraction of RNA from biological material by a sorption method, synthesis of cDNA on an RNA matrix by single-step formulation with addition of internal and positive control samples of the multiplex reverse transcription reaction and polymerase chain reaction with 45 amplification cycles with real-time detection using oligonucleotide primers specific for the viral pathogen region of the fluorescent labelled probe and control samples. Fragment of genome of type parainfluenza virus exciter has the following nucleotide sequences: BPIF 5'-TCGACATCATTGAATTCATAC-3' - forward primer; BPIR 5'-TCAAGTTGGTAGATTGTCGTGC-3' - reverse primer; BPI-z 5'-JOE-TGCCGTGTTCTCTTGGGAGTCG-BHQ1 - probe.

EFFECT: invention extends the range of methods for detecting genome of parainfluenza virus 3 in cattle.

1 cl, 1 ex, 5 tbl

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RU 2 696 069 C2

Authors

Chernykh Oleg Yurevich

Sochnev Vasilij Vasilevich

Bannov Vasilij Aleksandrovich

Malyshev Denis Vladislavovich

Drobin Yurij Dmitrievich

Donnik Irina Mikhajlovna

Ivanov Arkadij Vasilevich

Lysenko Aleksandr Anatolevich

Krivonos Roman Anatolevich

Shevkoplyas Vladimir Nikolaevich

Koshchaev Andrej Georgievich

Kaloshkina Inna Muratovna

Kulakova Anna Leonidovna

Dates

2019-07-30—Published

2018-01-16—Filed